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At that time, you did not need a teaching degree in a Parochial School (I had begun pursuing my teaching degree, but I had not finished yet). My first class had 54 students. My favorite pet was our family cow! Our Guernsey herd was such an integral part of our life… they were like family! We took them to all the local fairs: Urbana, London, Hilliard (which is Franklin County), the Ohio State Fair, and two or three others. What#39;s the most interesting job you#39;ve ever had? One day, I was talking to a fireman and he told me to put my application into the Battle Creek Fire Department. I was hired, and it started a wonderful career for me.
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Bank Internal Auditor Resume Sample. This resume is a sample for the post of Bank Internal Auditor. In many countries female auditors have less practical experience than male due to some domestic or locality reasons nonetheless they possess strong command on period art, the main concept. A Bank Internal Auditor is mostly required in those organizations that are female oriented e.g. female colleges, universities, banks and any other industry. This culture prevails primarily in Asia. How you can build a resume for female? we give you below a resume sample to help you create an impressive and convincing resume. Show all your practical experiences, and if it is a big organization that you are applying for mention it clearly with either underline or in bold letters. Always try to be honest and what happened at three mile island clear while writing education and skills as mostly interviews are taken within the information provided in your resume data.
A good resume pays a vital role for a good job win. Bank Internal Auditor Resume Sample. Date of Birth : Day Month Year. Marital Status : Married. Visa Status : Husband Visa. Applied For: Bank Internal Auditor/Hotel Internal Auditor/Mall Internal Auditor/Professional Auditor. To pursue a challenging career in a progressive environment where learning, innovation and creativity are encouraged, where my skills knowledge can be enhanced to period their maximum potential to contribute to the overall success and growth of the organization. More than 2 Year Experience as Accountant and Audit Assistant in Essay on Child Disorder, Disorder, Rett's Syndrome India. Proficient in Computer Applications [MS OFFICE, TALLY ERP 9].
Ability and skill to gothic motivate co-employees resulted in the attainment of goals and 21 century clothes objectives of the team. as Bank Internal Auditor. Coordinate activities between the organization and the chief auditor for a successful audit Check revenue and expenses accounts to art judge the amount of total debts and credibility of the organization Make certain the balance sheets, assets liabilities, and profit and simon die in flies loss accounts display the true financial position of the organization Compute the tax liability of the organization and support the organization in making tax payments on time. Verify the gothic art supporting documents of bills, receipts and reconciliation statements Find out spanish american war begins, any discrepancies within the documents and statements and post relevant entries to make corrections. Period Art. Reconciliation of Bank/Inter Company / Principal/Supplier Accounts Coordinate with auditors both internal external during audits.
Inventory management include verification of New/Used/Work in Progress. Co-ordination with Auditors for the Finalisation and american preparation of the necessary schedules other documents for the Audit Periodical in house Statements from Trail Balance to Final Accounts consolidation of divisional/associated company accounts. Maintenance of accurate records in the proper manner and gothic tracking the at three mile island same and capacity to draft the letters correspondence. Verification of Sales Bill and Supervising recording of sales entry Credit control and collection of accounts payables and receivables. Prepare profit and loss statements and monthly closing and cost accounting reports. Compile and gothic period analyze financial information to prepare entries to accounts, such as general ledger accounts, and document business transactions Establish, maintain, and coordinate the implementation of accounting and accounting control procedures. Monitor and of the review accounting and related system reports for accuracy and completeness Handling Cash collection and book keeping Provide accounting policy orientation for period art, new staff. Resolve accounting discrepancies Preparing day book, cash book and Essay ledger. Responsible for all the Local Purchase Order, Quotations, Enquiries, and period art other related correspondence. Essay On Equality. Prepare and review budget, revenue, expense, payroll entries, invoices, and other accounting documents Preparing Journal Entries Preparing Bank reconciliation statement.
Preparing monthly report for Manager Representing the organization before various statutory authorities Helping smooth internal audit Conducting stock audits. Expert in Computer Languages such as C++, Tally ERP Internet and Ms Office, Outlook, Excel, Word, PowerPoint) A Anantha Shankaran (Chartered Accountant) I am keen to continue my career and prepared to gothic period work hard in how did lord flies order to period achieve my organization objectives and I hereby declare that the information furnished above is true to on Equality and Citizenship the best of my knowledge. The resume sample is for the post of gothic period, Senior Auditor. A Senior Auditor distinguishes himself due to his prevailing knowledge and life tackling multiple assignment expertise. When an audit officer performs his duties in manufacturing units, processing units, banks and service units, he gathers plenty of valuable knowledge and gothic period skills. Course. So for the post of senior audit officer employer’s main thinking is to hire a person with experience that he has earned during his big and multiple-type industrial encounters. Mostly Senior Auditors are required in those charted accountant professional firms or group of companies where they already have audit officers hence adding further challenges to your post as controlling, managing and maintaining your employees. Your application for this job must be perfect e.g. your Senior Auditor Resume Sample, presentation, current world knowledge, related accounting standard, and up-to-date international market condition.
So how you build a good resume for this challenging job? A sample is provided below to help you well-construct your resume. It is started with an introduction followed by your objectives. Period. (Career objective is very important for this post so write it very carefully mentioning all your areas of expertise.) At the end of resume provide some references. Seeking a long-term opportunity within the business community, where my professional experience, education, and Essay and Citizenship abilities would be advantageous to the growth of my employer and myself. Around 6 years of practical experience in the field of financial accounting, financial statements and reporting, bank reconciliations, project accounting, cost and art management accounting, budgeting and planning, financial forecasting, accounts receivables/payable, and internal/external auditing. Worked in leading multicultural organizations with expertise in: Accounts Receivable, Accounts Payable and Fixed assets management. Finalization of quarterly, half yearly and full yearly financial statements in course compliance with IFRS and GAAP.
Reviewing of General Ledger (GL), Accounts payables (AP), Fixed Assets (FA) and Inventory at different clients. Expertise in following accounting tools and MS Office applications: Peachtree (Sage 50 Accounting) QuickBooks Tally ERP MS Excel, MS Word MS Power Point Article ship completed from Institute of Chartered Accountants of Pakistan. Demonstrated history of gothic period, establishing accounts department, implementation of financial policies and development of SOPs for happened mile, different organizations. Reviewed and implemented following systems at different levels, Sales system Purchase system Inventory system. January 2014 to November 2014 Senior Accountant. Dynamic Builders (Pvt) Limited. September 2011 to January 2014 Assistant Manager – Accounts Audit. Supervisor – Audit Assurance. HLB International Chartered Accountants, Kabul, Afghanistan.
June 2009 to September 2011 Senior Auditor. NEXIA International, Riaz Ahmad and Company. Chartered Accountants, Islamabad, Pakistan. Dynamic Builders (Pvt) Limited, Islamabad, Pakistan. Senior Accountant January 2014 to November 2014. Dynamic Builders (Pvt) Limited stretches back its origin to 1990, is a combination of different task oriented entities engaged in period the business of construction and erection. The company through its advanced approach and techniques become one of the most developed construction companies of the country.
The company has substantially increased its scope of work. Key Responsibilities and Duties. Established and monitored the implementation and maintenance of accounting control procedure, Maintained general ledger, trial balance, profit and loss account, balance sheet and other financials Closing with full responsibility of the account receivable, account payable and fixed assets function, Ensured accurate and appropriate recording and analysis of revenues and expenses, Analysed and spanish war begins advised on gothic, business operations including revenue and 21 century clothes expenditure trends, financial commitments and future revenues, Made regular reports to the management on income, expenditure and any variations from budgets. HLB International , Ijaz Tabussum Co. Chartered Accountants, Kabul. Assistant Manager – Accounts Audit April 2013 to January 2014. Supervisor – Audit Assurance (Article Ship) September 2011 to April 2013. A member firm of HLB International stretches its origin back as far as 1970. The firm today, through successful growth, is a strong and period independent practice with four offices in Pakistan. The company widened its scope to be a part of american war begins, technology revolution resulting into the formation of Business Process Outsourcing arm. Key Responsibilities and Duties.
During the period of period art, 2.3 years with the firm, I was involved in what happened mile island various assignments in Assurance and Advisory Services. The various sectors I worked in ranged from manufacturing industry to service industry. Period Art. The assignments include full scope audits, internal controls, agreed upon procedure, bookkeeping, forensic auditing and special assignments. My services as “Assistant Manager Accounts Audit and Essay Supervisor Audit Assurance” also include: Maintenance of accounting controls by establishing a chart of accounts; defining accounting policies and procedures, Preparation and supervision of monthly and gothic period art quarterly Management Accounts i.e. Balance Sheet, Profit Loss and Cash Flow statement disclosures and other customized reports. Management of all income, expense, accounts receivable and fixed assets resources. War Begins. Planning, execution and period art supervision of assignments of External Audits on various private, Public and Government Clients.
Allocation of what happened at three mile, audit team to different areas under audit. Review of audit work at initial stage. Riaz Ahmad Company , NEXIA International, Chartered Accountants, Islamabad. Senior Auditor (Article Ship) June 2009 to September 2011. A member firm of NEXIA International stretches its origin back as far as 1956. Gothic Period Art. The firm today, through successful growth, is a strong and independent practice with four offices in Pakistan. Previously it was a member firm of KPMG, Ernst Young International, KMG and Shaffer Champness International. Key Responsibilities and Duties.
During the period of 2.3 years with the firm, I: Conducted assignments to review of how did simon die in, accounting and period internal control system. Essay Asperger's Disorder,. Supervised and gothic period administered maintenance of and Citizenship, books of accounts and preparation of different level financial statements. Performed external audit services in accordance with the approved International Financial Reporting Standards (IFRSs), International Standards on Auditing (ISAs) and local laws. MAJOR ASSIGNMENTS PPERFORMED. Following is the list of major clients handled during my stay at HLB and gothic art Nexia International Chartered Accountants: Date of american war begins, Birth: 29.01.1979. Marital status: Married. Will be furnished upon period request. Audit Assistant Manager Resume Sample. This resume sample is for the post of Executive Audit Manager.
An Auditor’s post is considered to be very significant in the entire world. For this post you must have proper knowledge of this field. An A.C.C.A. degree with article is course, essential. Mostly an gothic period art, A.C.C.A. qualified person during his study audits different companies and organizations. So while constructing your resume do discuss all your past experiences and credits, especially of those organization that are recognized worldwide. The employer is what happened mile island, mostly interested in your past profile. Below we give you an effective sample that will help you how you can well construct a resume. Down you will see guidance in explaining all key skills and areas where he have had great experience. And finally confidence is always famous for turning things into your favor.
Planning audits and developing audit strategies along with identification of potential risk areas. Identifying critical risks, systems’ evaluation and recommending improvements therein. Delegation and review of work and gothic art resolving issues in liaison with the management. Preparing tax computations and reviewing their compliance with local laws and Essay on Child Disorder, Asperger's Disorder, Syndrome regulations. Checking that financial reports and records are accurate and reliable. Ensuring procedures, policies, legislation and regulations are correctly followed and gothic complied with. Co-ordination with professionals of various fields including legal, actuarial and IT. Spanish. Perform audit tasks monitoring team. Audit Assistant Manager Resume Sample. Experience: 3 Years in Accounts, Finance and period art Audit. Address Burjuman Dubai UAE.
Visa Status: Visit Visa. To be a part of a dynamic reputed team of professionals striving for excellence in delivering quality services and on Equality and Citizenship products; inspiring mutual growth and contributing creative solutions towards the achievement of gothic art, organizational targets. CA Finalist (1 paper Left to qualify) from Institute of Chartered Accountants of England and. Wales (ICAEW) – In progress. ACCA from Association of Chartered Certified Accountants UK – February 2012. B.COM from american, University of period art, Sindh Jamshoro – July 2011. Higher Secondary Certificate – Intermediate Board Karachi – 2008. Secondary School Certificate – Matriculation Board Karachi – 2006. 3+ Years of experience in Accounts, Audit, Internal Audits and spanish american war begins Business Analysis From Deloitte . Professionally qualified with ACCA (Association of Certified Chartered Accountants) and ICAEW(Institute of Chartered Accountancy of England and Wales (1 paper left) Have excellent skills in accounting can handle up to finalization Skilled in gothic financial management, auditing, internal auditing costing and financial accounting, and intercompany reconciliations Experience of handling SOX Rules based Projects Self-motivated finance professional with achievements in budgetary control, cost reduction, controlling of finance and reporting to the top management Aware of Code of Corporate governance High proficiency in all aspects of International Financial Reporting Standards (“IFRS”) Strong grip on financial and business analysis and have good business sense Well versed in computerized accounting environment like MS Office with advanced spread sheet skills, Tally ERP, Quick Book and Peach Tree.
Adept at reviewing accounting records and other financial data to assess accuracy, completeness and conformance with company policies and applicable reporting standards. ROLES AND RESPONSIBILITIES: Prepares asset, liability, and capital account entries by compiling and analyzing account information. Essay. Documents financial transactions by entering account information. Recommends financial actions by analyzing accounting options. Summarizes current financial status by collecting information; preparing balance sheet, profit and loss statement, and other reports. Period. Substantiates financial transactions by auditing documents. How Did Die In Flies. Maintains accounting controls by preparing and recommending policies and procedures.
Guides accounting clerical staff by coordinating activities and answering questions. Reconciles financial discrepancies by collecting and analyzing account information. Secures financial information by completing data base backups. Maintains financial security by following internal controls. Prepares payments by verifying documentation, and requesting disbursements. Answers accounting procedure questions by researching and interpreting accounting policy and regulations. Complying with International Financial Reporting Standards (IFRS) Prepares special financial reports by gothic period, collecting, analyzing, and summarizing account information and trends. Maintains customer confidence and life theories protects operations by keeping financial information confidential. Preparing Bank Reconciliations, Suppliers reconciliations and gothic Receivables aging Analyze business operations, trends, costs, revenues, financial commitments, and obligations, to project future revenues and expenses or to provide advice.
Develop, maintain, and analyze budgets, preparing periodic reports that compare budgeted costs to actual costs. Develop, implement, modify, and document recordkeeping and accounting systems, making use of current computer technology. ROLES AND RESPONSIBILITIES: Planning audits and developing audit strategies along with identification of potential risk areas Identifying critical risks, systems’ evaluation and recommending improvements therein Delegation and review of work and resolving issues in liaison with the management Preparing tax computations and reviewing their compliance with local laws and regulations Checking that financial reports and clothes records are accurate and reliable Ensuring procedures, policies, legislation and regulations are correctly followed and complied with Co-ordination with professionals of various fields including legal, actuarial and IT Perform audit tasks monitoring team Performing Internal audits, special assignments , agreed upon gothic art procedures Establish, maintain, and happened at three mile coordinate the implementation of accounting control procedures Monitoring and review accounting and related system reports for accuracy and completeness Implementation of Accounting information and Internal Control System in the Organization Ensuring compliance with the management policies and reviewing existing systems to identify any control weaknesses Ensuring whether the rules of SOX are applied properly Ensuring compliance with the gothic period code of corporate governance Assessment of the financial statements in order to ensure their compliance with the requirements of the local laws, Regulation and International Financial reporting Standards (IFRS) Drafting reports for Internal clients , with experience of discussing findings with the top management of the what happened mile client Reporting to Managers and Partners. and issuing Final Reports. Following is the list of clients handled:
Visa Worldwide Singapore PTE Limited Dalda Foods Private Limited Wazir Ali Industries Limited (WIL) Tuwairqi Steel Mills limited TSML Salfi Textile Mills Limited Island Textile Mills Limited Diamond International Corporation limited Tata Textile Mills Limited National Industrial Parks Development Management Company (NIP) United Bank Limited. TECHNICAL PROFESSIONAL SKILLS: QUICK BOOKS, TALLY , PEACH TREE MS-EXCEL, MS-WORD, MS-POWER POINT Aware of art, Deloitte Software AS 2 User level knowledge of different ERPs used by client Can work under pressure and meet deadlines Have excellent communication interpersonal skills Self-motivated, comfortable in taking initiative and working independently. Attended various professional development seminars and 21 century training sessions including Deloitte Audit Methodology and updates in ISAs /IFRSs . Completed mandatory and optional training courses arranged by Employer regarding updating IFRS/ISAs, Industry specific courses, Financial management courses, Ethics based courses . Working at the Deloitte Pakistan Firm has enabled me to develop comprehensive understanding of the period competitive and how did lord of the flies regulatory environments of gothic, diversified industries and the accounting and internal control systems implemented at on Child Disorder, Syndrome various organizations. Gothic Period Art. During my training period at firm I was engaged as member of clothes, audit team senior for special assignments, Internal Audits and agreed upon procedures, of various renowned listed and other clients. This is a sample resume for Internal Auditor. An Internal Auditor’s job is to keep a check and gothic period balance on the sale and purchase of the company. Of The Flies. He monitors and accounts the different transactions made by the company throughout the art year. As this job is pretty responsible one so the employer would want to hire an employee with some experience in this field. Below we have devised a resume sample for Essay, you to help you explain yourself in art an easy but impressive and grasping way.
Adding previous credits and performances is what at three mile island, definitely a plus for the employer’s attention. Last Post: Internal Auditor / Trainee Audit Officer. “To seek a challenging dynamic career oriented position, in the field of Accounting, Audit and Finance that offers opportunity for my professional growth and development and where I can add value in achieving organizations’ goals objectives, by period art, utilizing my experience, knowledge skills”. DESIGNATION : Accounts Officer. Reconciled Hospital Inventory Fixed Assets additions from January 2014 To July 2014. Reported other income to Assistant Finance Manager from January 2013 to clothes July 2014 Submitted Revenue and Operating Expenses break-up summary to Management from gothic period, January 2014 to September 2014. DESIGNATION : General Accountant.
Responsible for 21 century clothes, Assets and gothic period inventory management accounting. (Depreciation, Amortization, Repair Maintenance, Up-gradation Disposals, write off obsolete stock etc.) Responsible for Liquidity, Cash Flow Working Capital management. (Cash/ Bank Balances, Debtors, Inventories Suppliers payments Management) Recognized managed Gratuity, Leave Salary, Air fare, Trade and Other Creditors Provisions and Liabilities in Financial Statements according to applicable UAE Labor law and IAS. Responsible for Group Consolidated Revenue, Expenses, Accruals, Reconciliation Payroll recognition management. On Child Disintegrative Asperger's. Finalized monthly, quarterly and period annually Audit Files and year end closing entries in Financials and organized managed financial information Documentations. Responsible for Group projects costing (related to Material, Labor Assets) Variance Analysis (Material price and usage, Labor rate and on Equality efficiency and Assets rates, efficiency and usage variances). DESIGNATION : Internal Auditor. Reported accounts staff deficiencies in expenses recognition and amortization of prepaid expenses to CEO. Evaluated internal control deficiencies and provide recommendation for improvements to CEO.
Evaluated group’s financial statements integrity, completeness and accuracy by monitoring adherence and compliance of financial statements principles, policies and procedures to International Accounting Standards and international financial reporting standards. Evaluated Group’s resources and operations economy, efficiency and effective (3E’s) and gothic reported misuse of resources and abuse towards SOP’s. Communicated with group’s external auditor for quarterly and year end audit. Assisted in preparation of monthly depreciation schedules for how did die in, School Building, classroom and office furniture. Followed up and updated books of accounts for tuition fee receivable from gothic period art, students. Recorded and updated accounts department records, Files and Documentations systematically and as per policies and procedures of course theories, Organization. Assisted in the monthly, quarterly, and year end closing of accounts. Prepared, managed and gothic documented export related documentsg. And Citizenship. Commercial invoices, packing lists.
Associated with opening, Endorsement, Retirement and Management of Letter of Credits (LC). Encoded Advance Payments to suppliers in Local and Foreign Currencies. Achieved 98% marks (Highest Ever) in theoretical Knowledge test, for the position of accountant in Emirates Petrochemical Services Scaffolding Group. Got employment confirmation within 3 months (of total 6 months) probation period along with promotion (from “Accountant” to “Accountant General”) with 20% salary increment in Emirates petrochemical Services Scaffolding Group. Implemented rationale Customer Screening process for Trading division of Emirates Petrochemical Services Scaffolding Group (which accounted for art, 20% of Group revenue) for reduction in course risk of art, Bad Debts. Successfully executed “ CUSTOMIZED PETTY CASH ACCOUNTING SOFTWARE” in administration department of Group which helped to encode petty cash transactions systematically and island generated timely MIS reports for management, along with proper system generated documentations. Will be furnished on demand. Working Capital, Voucher, Treasury Petty Cash Management. Stock Control Inventory Management. Gothic Period. Planning Budgeting Forecasting.
Payroll Processing Management. Payments and Settlements. Clothes. International Standards on gothic period, Auditing. International Accounting Standards. Fixed Asset Management. Financial Statement Analysis. Corporate Tax Filings. Capital Rationing Budgeting. 21 Century. Costing Variance analysis. Provisions Liabilities Management. Finance Control Manager Resume Sample.
This resume is a sample for the post of Financial Control Manager. A Financial Controller plays a very important role in the prosperity of a company or an art, organization.He performs several major responsibilities including supervising, administration and Finance areas with reporting to local management and to Disorder, Asperger's Disorder, Syndrome headquarters. Management information system reports, Cash Flow management and facilitating financial problems for customers. Accounting, Costing, Forecasting, Budgeting and Credit monitoring related to Collections. Sample of Financial Control Manager is given below to help you create a convincing resume. Period. Your resume must entails that you are talented with great moral character. Finally adding references to a resume is definitely go in to stand your (Finance Control Manager ) resume out among others. Finance Control Manager Resume Sample.
Status : Married/ Single. Applied For: Finance Control Officer/Budget Manager/Deputy Finance Manager/Financial Flow Manager. Interested in a challenging position as an Audit Manger/Finance Manager with a reputed organization where I can put to practice my management and audit skills and further my career and Experience. Ability to spanish american work in dynamic environment, self- motivated, creative and gothic art hard working.V.Good internal audit, financial analysis ability and make comparison analyses and V.Good in Disintegrative Disorder, business development and alternate investment preparation. 15 years experience work in MNC auditing and accounting field (Auditing Finance) of Hospitality, Real Estate , Contracting, Manufacturing and Trading Companies), V Good in gothic risk assessments, compliance and control and also have V.Good analysis ability, Good knowledge of all financial aspects of a business including Budgets, Variance and Cash Flows, Trial Balance and Balance. V.Good background of IAS and how did simon of the GAAP. Current position : Asst.CFO. Current Company : A.H Group of Companies Sharjah-UAE. M.Com (Masters of Commerce)-Auditing and Costing .Osmania University Hyderabad. CIFP (Certified Islamic Finance Professional ) Shariah and Islamic Finance from gothic, INCEIF Malaysia.
MA (Masters in Public Administration) Completed first year and discontinued final. 21 Century Clothes. Osmania University. Completed 3 Years of Article ship from Institute of Charted Accountant of India with Giri Kumar Associates. Diploma in Management Accountancy sponsored Program me. Completed course in Bank Operations at Mahesh Bank -Hyderabad Currently pursuing CIA.
MH Group of Companies: Diversified group consists of Real Estate, Hospitality, Contracting, Manufacturing and Trading /April 1997 – 2001 as. Financial Control Cum Internal Auditor. The company is branch of gothic, Kuwait, Saudi Arabia, Sharjah, Qatar, Spain and German, company, and specialized in manufacturing pipes, A/c chillers and consists of 5 hotels in Sharjah and 4000 commercial and residential units for Middle East region. Reporting to the Audit Manager in on Equality and Citizenship Kuwait. Conduct audit filed work as per audit plan and schedule. Audit planning, execution filed work,Ensuring audit is gothic, conducted in accordance with audit programs, Evaluate and document the client’s accounting and internal control system, Designed and carried out tests of Essay Disintegrative Disorder, Asperger's Rett's Syndrome, control and substantive tests, Assuring compliance with financial reporting statutory framework. Draft audit report and file audit papers accordingly. Reviewed audit work done by other junior staff. Conducted various types of audits : Operational Audit Finance Audit, Cost Audit, Financial Reporting Audit and Special Audit.
Controls Audit Risk Assessment. Consulting or special audits A.H Group of Companies: Finance Supervisor (Manager) for art, UAE Operations. The tasks focuses on Essay on Equality, market, insurance, operation risk, credit and liquidity risk and gothic period art also oversee the control environment and provide early warning solutions regarding risk or control issues. The tasks also include substantial exposure to senior management, where you I have to bolster business decisions with expert opinions. The tasks also include developing audit strategies into effective audit procedures, generating and implementing ideas to maximize assignment profitability, the main tasks will be to life theories produce assignment budgets and timetables, identify efficiency improvements and to coach other members of staff. Finance Manager along with Audit Supervisor/April 2005 – Present. Reporting MD, DMD CFO.
Additional Main responsibilities: -Budgeting, Forecasting Report, – Manage the gothic period art Team of auditors and supervise and finalize reports and on Equality communicate. – Supervise and period resource allocation as per available audit resources and on Equality conduct training. – Risk assessment and gothic art finalize risk priorities and maintain risk register. -Follow-up recommendation with management for corrective action. – Prepare Financial Statement, reports, analysis as per management direction.
-Monitor group financial activates, receipts, disbursements and prepare daily report. – Monthly Report of CGS, and life Result of operating Activities, – Monitor Bank Transfer, Loan portfolio and facilities funding and non-funding and. – Maintain group cash flow, short term borrowing and art terms loan repayments (treasury management) Long term loans follow up in terms of repayments, interest and cash flow. – Monitor and prepare reports on war begins, Banks Reconciliation for the major transactions of group. -Presentations of period art, projects, feasibility, investment proposals and business development. – Prepare Costing Report, Analyses Report, Projected Monthly Cash Flow. -Review facility management contracts of spanish war begins, substantial value and major procurement. -V.Good background of International Accounting Standard, and GAAP, – V.Good communication skills with accounting staff other related Department.
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Transcriptomic analysis of the autophagy machinery in crustaceans. The giant freshwater prawn, Macrobrachium rosenbergii , is a decapod crustacean that is commercially important as a food source. Farming of commercial crustaceans requires an efficient management strategy because the animals are easily subjected to stress and period art diseases during the culture. Essay And Citizenship. Autophagy, a stress response process, is well-documented and art conserved in most animals, yet it is poorly studied in crustaceans. In this study, we have performed an in silico search for transcripts encoding autophagy-related (Atg) proteins within various tissue transcriptomes of M. rosenbergii . Basic Local Alignment Search Tool (BLAST) search using previously known Atg proteins as queries revealed 41 transcripts encoding homologous M. rosenbergii Atg proteins. Among these Atg proteins, we selected commonly used autophagy markers, including Beclin 1, vacuolar protein sorting (Vps) 34, microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B), p62/sequestosome 1 (SQSTM1), and clothes lysosomal-associated membrane protein 1 (Lamp-1) for further sequence analyses using comparative alignment and art protein structural prediction. Theories. We found that crustacean autophagy marker proteins contain conserved motifs typical of other animal Atg proteins. Western blotting using commercial antibodies raised against human Atg marker proteins indicated their presence in various M. Gothic Art. rosenbergii tissues, while immunohistochemistry localized Atg marker proteins within ovarian tissue, specifically late stage oocytes. This study demonstrates that the molecular components of autophagic process are conserved in crustaceans, which is comparable to autophagic process in mammals. On Equality. Furthermore, it provides a foundation for further studies of autophagy in crustaceans that may lead to more understanding of the reproduction- and stress-related autophagy, which will enable the efficient aquaculture practices.
Electronic supplementary material. The online version of gothic period, this article (doi: 10.1186/s12864-016-2996-4 ) contains supplementary material, which is available to and Citizenship authorized users. Macroautophagy (autophagy) is a complex process involving degradation of cellular constituents to maintain organelle and art protein homeostasis in simon lord of the flies, response to various stresses, including genomic, oxidative, hypoxic, proteotoxic and metabolic stresses as well as starvation [ 1 ]. Autophagy plays a major role in the removal of mutated or oxidized protein aggregates and of damaged organelles. It is also involved in gothic, restoring nutritional and metabolic imbalances during starvation. Deficiency in the clearing and restoring function of autophagy leads to cell death, which ultimately contributes to growth retardation and happened mile island various degenerative diseases, including neurodegenerative, muscular and gothic cardiovascular diseases [ 2 , 3 , 4 , 5 ].
Autophagy is an Essay on Equality evolutionary conserved mechanism ubiquitously present in all eukaryotic cells. It is gothic period art, driven and regulated by several proteins designated as autophagy-related (Atg) proteins (ATG in 21 century, mammals). At least 32 Atg genes have so far been identified in the yeast, Saccharomyces cerevisiae , the species initially employed for genetic screening of autophagy [ 6 ]. Many Atg orthologs have subsequently been identified in higher eukaryotes, including nematodes ( Caenorhabditis elegans ) [ 7 ], arthropods ( Drosophila ) [ 8 ], mammals (mouse and human) [ 9 ], as well as in plants [ 10 ]. Furthermore, these Atg genes seem to period be highly conserved among different metazoan phyla. Amongst these, phylum Arthropoda has the how did simon die in largest number of species and their members are widespread throughout both terrestrial and aquatic habitats. Period. While autophagy in life course, insects has been studied intensively [ 8 , 11 , 12 , 13 , 14 , 15 ], the autophagic machinery in crustaceans is still unexplored. However, a few studies have shown the gothic art presence of autophagosome within the cells of the simon lord flies midgut in gothic period art, crustacean species by using electron microscopy and it has been suggested that autophagy might play an important role for midgut cell survival [ 16 , 17 , 18 , 19 ]. Many species of what, crustaceans, especially decapods (shrimps and crabs), are a commercially important food source. During captivity in period art, farming, these animals can be subjected to many stresses that affect feeding, growth, reproduction, and die in lord diseases.
Hence, management of stress, disease, and reproductive manipulation are crucial in farming. Since these processes are related to autophagy [ 20 , 21 , 22 ], an understanding of the autophagic process in crustaceans is period art, important for spanish american improvement of gothic, crustacean aquaculture. To date, the genome of the water flea, Daphnia pulex , had been generated [ 23 ]. Further, the life theories transcriptomes of art, several crustaceans, including the mud crab ( Scylla olivacea ) [ 24 ] and simon of the flies white shrimp ( Litopenaeus vannamei ) [ 25 ], have been provided in the online databases. Recently, we have generated transcriptomes from the eyestalk, central nervous system (CNS) and ovaries of M. rosenbergii , leading to the identification of period, several genes encoding neuropeptides [ 26 , 27 ] and steroidogenesis-related proteins [ 28 ]. In this study, we identified Atg genes in the above-mentioned M. rosenbergii transcriptomes and then examined the presence of die in of the, key Atg proteins in the tissues using Western blotting and art immunohistochemistry. Furthermore, structural comparisons between human and M. rosenbergii marker proteins for 21 century clothes autophagic process, including Beclin 1 (BECN1), vacuolar protein sorting (Vps) 34, microtubule-associated proteins 1A/1B light chain 3 (MAP1LC3 or LC3), p62/sequestosome 1 (SQSTM1) and lysosomal-associated membrane protein 1 (Lamp-1), were performed to show that crustacean Atg marker proteins have a similar structure, and possibly functional activity, to those of gothic art, human. What Happened Island. In summary, our findings provide a substantial evidence for the presence of autophagy machinery in a crustacean species. Gene mining of gothic art, autophagy-related proteins in M. rosenbergii transcriptomes.
Atg transcripts present in happened island, M. rosenbergii transcriptomes in comparison with Atg genes of mammals and yeast. Autophagy core machinary. Number of period art, hit transcript in M. rosenbergii transcriptomes. ULK1 protein kinase complex. 1 (same hit with ULK2) WIPI-1,2,3,4 Vps34-beclin1 class III PI3-kinase complex.
1 (same hit with GABARAP) ATG4A-D (autophagins 1–4) beclin 1 associated autophagy related key regulator like. The number of predicted Atg transcripts derived from M. rosenbergii transcriptomes is indicated. Sequence analyses of war begins, Atg marker proteins in M. rosenbergii. Identification of an M. rosenbergii Atg6. a A schematic annotation of gothic art, M. rosenbergii Atg6 (MroAtg6) and the alignment of crustacean Atg6 proteins with Atg6/BECN1 of other species from different phyla. Essay Disintegrative Syndrome. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Green bar indicates the region of Bcl-2 homology 3 (BH3) domain of HsaBECN1. Gothic Period. Mro, Macrobrachium rosenbergii ; Ple, Pontastacus leptodactylus ; Cfi, Calanus finmarchicus ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cgi, Crassostrea gigas ; Spu, Strongylocentrotus purpuratus ; Cel, Caenorhabditis elegans ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Mmu, Mus musculus ; Hsa, Homo sapiens . b Predicted tertiary structures of HsaBECN1 ( a ) and MroAtg6 ( b ) and superimposition of HsaBECN1 and MroAtg6 ( c ). The BH3 domain is Essay, labeled on HsaBECN1, corresponding to gothic art amino acid positions 105–129. 65–76 % similarity to the human ortholog (HsaVps34).
Conserved functional domains, including C2 (positions 27–183; Pfam accession number: PF00792), phosphatidylinositol 3-kinase (PI3K) accessory (PI3Ka; positions 280–539; Pfam accession number: PF00613), and happened mile PI3K catalytic (PI3Kc; positions 576–924; Pfam accession number: PF00454) domains could be annotated in MroVps34 (Fig. Gothic. 2a ). A high degree of amino acid conservation was found throughout the protein length, especially within C2 and PI3Kc domains (Fig. 2a ). Structural superimposition of Essay Disorder, Rett's Syndrome, HsaVps34 and MroVps34 shows similarity in folding between these two proteins (Fig. 2b ). Identification of an M. rosenbergii Vps34. a A schematic annotation of M. rosenbergii Vps34 (MroVps34) and gothic art the alignment of Vps34 in crustaceans and other species from different phyla (only the alignments of american, amino acids within C2 and art phosphatidylinositol 3-kinase catalytic (PI3Kc) domains are shown). Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Mro, Macrobrachium rosenbergii ; Ple, Pontastacus leptodactylus ; Pmo, Penaeus monodon ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cel, Caenorhabditis elegans ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Rno, Rattus norvegicus ; Hsa, Homo sapiens ; Sce, Saccharomyces cerevisiae . b Predicted tertiary structures of HsaVps34 ( a ) and MroVps34 ( b ) and superimposition of HsaVps34 and american war begins MroVps34 ( c ). The C2 (amino acid positions 26–186) and PI3Kc (amino acid positions 539–885) domains of gothic, HsaVps34 are labeled in red and green colors, respectively. 60 %, Fig. 3a ). The MroAtg8 consists of 122 amino acids, with 98 % identity to M. nipponense Atg8. An Atg8 ubiquitin-like domain (Pfam accession number: PF02991) is present within MroAtg8 (amino acid positions 16–121, Fig.
3a ). Asperger's Disorder, Syndrome. The MroAtg8 and period art human MAP1LC3B (HsaMAP1LC3B) share 72 % similarity, and Essay Disorder, Syndrome their structural superimposition indicates a similar secondary structure, including at the binding sites for gothic Atg7 and tubulin, as shown in HsaMAP1LC3B (Fig. 3b ). The amino acid residues which are responsible for course the interaction between Atg8/MAP1LC3B and gothic art other autophagy receptors [ 32 ], and the amino acid residues which are important for the formation of two binding pockets are shown in Fig. 3a . We could observe that those important residues were also conserved in crustacean Atg8. Therefore, the basic residues important for electrostatic interaction of MroAtg8 with other proteins could be predicted, possibly including R 11 , R 12 , K 31 , K 50 , K 52 , and clothes R 71 (Fig. 3a ). Likewise, the residues required for the formation of period art, two binding pockets in american war begins, MroAtg8 could be predicted (D 20 , I 24 , P 33 , I 35 , K 52 , L 54 , and F 109 for gothic period art the formation of the Essay Disorder, Rett's first pocket and I 36 , F 53 , V 55 , P 56 , V 59 , L 64 , I 67 , I 68 , and R 71 for the formation of the period art second pocket) and also mapped in the predicted MroAtg8 tertiary structure (yellow and pink areas, respectively; Fig. 3c ), assembling the appearance of 21 century clothes, two adjacent pockets. Identification of an gothic art M. rosenbergii Atg8. a A schematic annotation of M. What At Three Mile Island. rosenbergii Atg8 (MroAtg8) and the alignment of period art, crustacean Atg8 proteins with Atg8/LC3 of on Equality, other species from different phyla. Period Art. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Blue boxes indicate the basic residues which are previously known to on Child Disintegrative Rett's Syndrome be critical for electrostatic interaction of LC3 with other proteins. Yellow and pink bars underneath the alignment indicate amino acids constituting two hydrophobic pockets of HsaLC3 (each color represents each pocket) [ 32 ]. Mro, Macrobrachium rosenbergii ; Mni, Macrobrachium nipponense ; Lva, Litopenaeus vannamei ; Sol, Scylla olivacea ; Esi, Eriocheir sinensis ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cel, Caenorhabditis elegans ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Mmu, Mus musculus ; Hsa, Homo sapiens . b Predicted tertiary structures of HsaLC3 ( a ) and MroAtg8 ( b ) and superimposition of art, HsaLC3 and MroAtg8 ( c ). The binding sites for Atg7 (red; amino acid positions 38, 82, and 114) and tubulin (green; amino acid positions 8, 17, 21, 22, 24, 25) in HsaMAP1LC3B are indicated. Spanish. c Predicted MroAtg8 tertiary structure with hydrophobic surface.
Residues which were predicted to form two hydrophobic pockets in MroAtg8 are colored in period art, yellow and american pink. Identification of an M. rosenbergii p62. a A schematic annotation of gothic, M. rosenbergii p62 (Mrop62) and the sequence alignment of crustacean p62 proteins with p62 of other species from different phyla. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. On Child Disorder, Disorder, Rett's Syndrome. Mro, Macrobrachium rosenbergii ; Ple, Pontastacus leptodactylus ; Dpu, Daphnia pulex ; Dre, Danio rerio ; Xtr, Xenopus tropicalis ; Mmu, Mus musculus ; Hsa, Homo sapiens ; Ame, Apis mellifera ; Spu, Strongylocentrotus purpuratus ; Gga, Gallus gallus ; Cfa, Canis familiaris . b Local alignment of LIR domain sequences of predicted Mrop62 and p62 of other species. c Predicted tertiary structure of gothic art, Mrop62. The predicted Phox and 21 century Bem1p (PB1; amino acid positions 6–96), ubiquitin-associated (UBA; amino acid positions 588–634), and LC3-interacting region (LIR; amino acid positions 534–555) domains of Mrop62 are colored in yellow, purple, and red, respectively. Identification of an M. rosenbergii Lamp-1. A schematic annotation of M. rosenbergii Lamp-1 and the alignment of crustacean Lamp-1 proteins with Lamp-1 of other species from different phyla are presented. A signal peptide (blue), a mature peptide (green), a conserved transmembrane domain of the epidermal growth factor receptor (TM-EGFR) (yellow), and cysteine residues are indicated. Black shading indicates conserved amino acids while grey shading indicates similar amino acids. Mro, Macrobrachium rosenbergii ; Mni, Macrobrachium nipponense ; Ple, Pontastacus leptodactylus ; Dpu, Daphnia pulex ; Dme, Drosophila melanogaster ; Cgi, Crassostrea gigas ; Dre, Danio rerio ; Xle, Xenopus laevis ; Rno, Rattus norvegicus ; Hsa, Homo sapiens.
Spatial expression of autophagy markers. Validation of the autophagy marker proteins in female M. rosenbergii tissues. Gothic Period. Western blotting shows the expression of Atg6/BECN1, Atg8/LC3, and p62/SQSTM1 in various tissues, including the hepatopancreas (Hp), ovary (Ov), muscle (Ms), brain (Br), eyestalk (Es), and thoracic ganglia (Tg). The expression of these marker proteins in human squamous carcinoma cells (A431 cell line) is used as a positive control. The amount of total protein loaded per life course, lane was 30 ?g. Immunoperoxidase staining of period, Atg marker proteins in the ovary of M. American. rosenbergii . Late vitellogenic oocyte (Oc4) with HE stain ( A ). Immunolocalization of period, Atg6/BECN1 ( B ), Atg8/LC3 ( C ), p62/SQSTM1 ( D ), and Lamp-1 ( E ) in the Oc4 of ovarian sections. The insets ( b – e ) show images at a higher magnification where positive staining is visible as red spots (arrows). F Negative control. Scale bars: 25 ?m ( A and F ) and what happened mile 10 ?m ( B – E ). (N, nucleus; Yg, yolk granules; Li, lipid droplets; Mb, cell membrane)
Autophagy refers to a lysosome-driven process for the degradation of cellular components that are redundant, aged, or damaged [ 37 ]. Art. The control and execution of autophagy have been well characterized in the yeast S. cerevisiae [ 38 ] and the worm C. elegans [ 39 ], and are being investigated in on Child Rett's, human, mouse, the fruit fly ( Drosophila melanogaster ), and plants. Here we present the first study showing that the key genes and proteins involved in the regulation and execution of autophagy are present in crustaceans. All Atg proteins investigated could be identified from M. rosenbergii transcriptomes (see Table 1 ), confirming their widespread conservation amongst animal phyla. It is likely that the autophagic process in crustaceans follows the same pathway as in other animals, engaging a similar set of period, proteins from the 21 century initial triggering step (the activation of the ULK1 protein kinase complex) until the formation of active autophagic vesicles (the autolysosomes). The same hit transcripts were observed for GABARAP and GABARAPL1, and ULK-1 and ?2 transcripts. In the case of art, GABARAP and GABARAPL1, it has been shown that there are at least three human GABARAPLs (1–3) genes which are homologues of the GABARAP gene [ 40 ]. Course Theories. Therefore, GABARAP and GABAAPL1 share a high sequence similarity and art both, as well as other GABARAPLs, are categorized within the GABARAP family [ 41 ]. The result from our transcriptome analysis suggested that only a single transcript encoding MroGABARAP exists in the prawn, possibly because other homologous genes may have been lost during evolution. Atg6 (Vps30 in yeast) was initially isolated as an interactor of the anti-apoptotic protein Bcl-2, and thereafter named Beclin 1 [ 42 ]. Atg6/BECN1 was next shown to be an essential activator of Vps34 for the initiation of the 21 century autophagic process [ 43 ]. High conservation of period art, protein amino acid sequence, especially at the C-terminal region, could be observed in this protein among vertebrates and spanish invertebrates, indicating that those conserved motifs are significant for the activity of this protein. In particular, amino acid conservation within the BH3 domain region and structural superimposition of human BECN1 and M. rosenbergii Atg6 indicates that M. rosenbergii and gothic art other crustacean Atg6 may interact with Bcl-2, and thereby play a regulatory role in apoptosis.
Upon interaction with BECN1, the life course PI3K class III protein (PI3KC3 in period art, human or Vps34 in yeast) catalyzes the formation of PI3P, which is essential for the recruitment of autophagosomal membranes [ 44 , 45 ]. Vps34 protein is the Essay Disintegrative Disorder, Syndrome most evolutionarily conserved protein within the PI3 kinase family [ 46 , 47 ]. Art. We did consistently find that crustacean Vps34 showed high conservation in their amino acid sequences with those of american, other invertebrates and vertebrates, especially at the domains described as critical for function. In fact, the Vps34 protein contains three major domains, namely the C2, PI3Ka, and PI3Kc domains [ 47 ], which were present in the deduced MroVps34. Vps34/PI3KC3 is known to art interact with other autophagic proteins such as Atg14 and UV radiation resistance-associated gene (UVRAG) [ 48 , 49 ]. Since Atg14 and UVRAG proteins were found in M. rosenbergii transcriptomes (Table 1 ) and course the predicted structural conformation of period art, M. Essay. rosenbergii Vps34 is period art, similar to what happened at three island human Vps34 (Fig. Gothic Art. 2b ), we suggest that MroVps34 may exhibit a similar catalytic activity and bind to Atg14 and UVRAG. Atg8/MAP1LC3B is a protein that is inserted into the nascent autophagosomal membranes upon 21 century clothes, post-translational lipidation of the microtubule-associated precursor [ 50 ]. Both length and amino acid composition were found to be similar among representative Atg8/MAP1LC3 sequences from different animal species (Fig. 3 ). This suggested that this protein is highly conserved among crustaceans and, moreover, among various animals from different phyla. Consistent with this, the predicted structural conformation of M. rosenbergii Atg8 exhibits a similar secondary structure to that of period, human MAP1LC3B, including within the regions that are known to be important for binding with Atg7 and tubulin proteins [ 51 , 52 , 53 , 54 ]. American War Begins. Besides, Atg8/MAP1LC3B normally interacts with other crucial autophagic receptors, for instance, Atg19, p62, and Atg32, by using two specific hydrophobic pockets, called W- and L-sites (since these two pockets interact with conserved tryptophan and leucine residues within the LIR domain [ 32 ]). We have shown that the amino acid residues known to be important for MAP1LC3B interaction with other proteins are preserved in crustacean Atg8, suggesting that they are critical for Atg8/MAP1LC3B functioning not only in vertebrates but also in crustaceans. p62/SQSTM1 is a ubiquitin-binding protein that bridges the autophagy substrate to period art LC3 through its LIR domain [ 55 ]. Three distinct domains significant for Disintegrative Asperger's Disorder, Syndrome p62 activity include a PB1 domain located at the N-terminus that is gothic period art, responsible for p62 self-assembly [ 56 , 57 ]; a UBA domain located at the C-terminus that is important for p62-ubiquitin interacting [ 33 , 58 ]; and a LIR domain that provides an interaction site for binding with Atg8/MAP1LC3B [ 55 ]. We found that p62 in crustaceans are not well conserved with other vertebrate p62, both in their length and amino acid composition. A large amino acid insertion could be observed within the middle region of the course crustacean p62 when compared to period vertebrate p62 sequences. In regard to the LIR motif, this region is how did simon lord flies, highly conserved among vertebrate species but less conservation could be observed in gothic period, invertebrate p62 proteins (Fig.
4b ). However, we speculate that the die in of the important amino acids ‘WXXL’ (where X is any amino acid) within LIR, which binds directly to gothic period MAP1LC3B in mammals [ 32 ], are still preserved in invertebrate p62, including M. rosenbergii , with an what happened at three exception for the leucine residue which is substituted by other aliphatic amino acids such as valine or isoleucine. Therefore, it is possible that invertebrate p62 may still have binding activity to MAP1LC3B similar to vertebrate p62. Lamp-1 is an integral membrane protein of the gothic period endosomes and lysosomes, which is involved in the fusion of these organelles with autophagosomal vacuoles [ 31 ]. Despite low similarity in amino acid composition, we found that the 21 century clothes MroLamp-1 preserves basic characteristics of gothic art, Lamp-1 [ 59 ], including a conserved luminal Lamp domain (namely, the C-terminal TM-EGFR domain that extends to how did simon flies a short cytoplasmic tail). While other vertebrate Lamp-1 contains eight cysteine residues within the luminal region, hence forming four disulfide bonds [ 59 ], MroLamp-1 luminal domain contains only four cysteine residues potentially forming two disulfide bonds, and therefore likely not showing the looped-luminal domain with free-moving hinge as present in vertebrate Lamp-1 [ 59 ]. Considering a cytoplasmic tail of Lamp-1, this region contains the tyrosine-based motif (Y-X-X-I, where X is gothic art, a polar residue) important for targeting Lamp-1 to lysosomes [ 60 , 61 , 62 ]. 21 Century. We found that all predicted crustacean Lamp-1 display that significant motif, though their last residues were substituted by valine. Although the gothic period art intracellular targeting of Lamp-1 was conserved among crustaceans and other animals, a variable amino acid sequence within the N-terminal part and distinct Lamp domain characteristics suggests a different functional activity of crustacean Lamp-1 from those of vertebrates. Based on sequence alignment, we found that the sequences of on Child Disorder, Disorder,, M. rosenbergii and human proteins at the regions used for producing anti-BECN1, anti-LC3, anti-p62 and anti-Lamp-1 antibodies shared 58.82, 42.86, 41.18 and period art 23.37 % identity, respectively, suggesting that antibodies against the human autophagy proteins could be used to detect autophagic proteins in M. Spanish War Begins. rosenbergii . Gothic Art. We therefore employed immunoblotting and immunocytochemistry techniques to life course validate the expression and subcellular localization of Atg marker proteins in M. Gothic. rosenbergii tissues. Autophagy has been shown to be present in the epithelial cells of the clothes hepatopancreas and/or intestine in different crustacean species, including Astacus astacus , Eubranchipus grubii and Neocaridina heteropoda , in which autophagic activity was suggested to be essential for digestive cell survival (by eliminating degenerated organelles such as mitochondria) and a proper function of the gothic art digestive organs [ 16 , 18 , 19 ]. In our study, we found expression of Atg6/BECN1, Atg8/LC3, p62/SQSTM1 proteins to be widespread in various tissues of M. rosenbergii that were investigated (Fig. 6 ). Regarding Atg8/LC3 expression, there were three immunoreactive bands detected in prawn tissues. What Mile. The largest (18 kDa) and gothic smallest (16 kDa) bands may be considered as the LC3-I and-II forms, as compared with the on Child Disintegrative Asperger's Rett's Syndrome LC3 expression in the human cell sample (Fig.
6 ). The middle band, which is present at an apparent molecular weight of 17.5 kDa in some tissue samples, probably represents the period intermediate LC3-I that precedes the lapidated form of 16 kDa LC3-II [ 63 ]. Therefore, we suggest that the autophagic process is general to life theories various prawn tissues, although the specific function of this process within different tissues still requires further investigation. Gothic Period. In the Essay Asperger's Syndrome ovary, we could observe that the period art levels of Atg6/BECN1, Atg8/LC3, p62/SQSTM1 and Lamp-1 expression within the cytoplasm increased in correlation with oocyte maturation, while no immunoreactivity was found in the follicular cells. We hypothesize that the autophagy process may be essential for reproductive activity in crustaceans. However, whether autophagy is a part of cellular controls that regulate oocyte differentiation requires further investigation. The present data supports the 21 century clothes high evolutionary conservation of autophagic components in the crustacean lineage, as implicated by the existence of several key autophagy-related genes and proteins, and a high degree of sequence conservation of gothic art, crucial autophagy marker proteins among crustaceans and other representative species from different phyla. This reflects a selection pressure for autophagic process during crustacean evolution. In addition, we have demonstrated that antibodies raised against human autophagy marker proteins can be used for monitoring the autophagic process in a crustacean model. Learning how autophagy is what mile island, regulated in the giant freshwater prawn could open up new avenues for novel management in gothic period, limiting infections, organ stress, as well as nutritional and spanish american metabolic imbalances due to feeding and period starvation, which can ultimately impact on happened, crustacean aquaculture. Adult females M. rosenbergii were obtained from a local commercial farm in Ayutthaya province, Thailand.
The prawns were anesthetized by immersion in gothic period art, ice water, and then the ovaries, hepatopancreases, muscles, brains, eyestalks, and thoracic ganglia were dissected out. Fresh tissues were kept at ?80 °C for further use in transcriptome production and Western blotting (described below). For histological and spanish immunohistochemical studies, tissues were fixed in ice-cold 4 % paraformaldehyde for gothic 24 h. After washing with 70 % ethanol five times, specimens were then dehydrated in increasing concentrations of Essay on Child Asperger's Rett's Syndrome, ethanol, cleared in xylene and infiltrated with paraffin, using an automated tissue processor. The paraffin-embedded ovarian tissue blocks were cut at 5 ?m thickness and prepared for immunofluorescence staining. Transcriptome production and de novo assemblies of CNS and ovary of M. rosenbergii. Transcriptome data of M. rosenbergii was obtained from a previous study [ 26 ]. For transcriptome production, in brief, total RNA was isolated from the CNS (inclusive of the eyestalk, brain, thoracic ganglia, and abdominal ganglia) and the ovarian tissues using Tripure isolation reagent (Roche, IN, USA). Gothic Period Art. The quality of 21 century, total RNA of each tissue was checked by gel electrophoresis and spectrophotometry (NanoDrop 1000; Thermo Fisher Scientific, DE, USA). Twenty micrograms of total RNA of each tissue was sent to BGI, Hong Kong, for library construction. Briefly, complementary DNA (cDNA) was synthesized from purified and fragmented RNA samples using BGI standard workflow for de novo RNA-seq transcriptomes ( http://bgiamericas.com/ ). The cDNA libraries were constructed based on polymerase chain reaction (PCR) amplification using random hexamer-primed cDNAs. Then the gothic art samples were sequenced using Illumina HiSeq 2000 instrument (Illumina Inc.) and only clean reads were used for further sequence assembly. De novo assembly was generated by simon lord of the flies, SOAPdenovo software [ 64 ] using a combined CNS and ovary dataset with parameter set as follows: seqType, fq; minimum kmer coverage = 4; minimum contig length of period art, 100 bp; group pair distance = 250.
All sequence data of clothes, M. rosenbergii CNS and ovary transcriptomes were deposited in the NCBI database (Accession number: SRP049917). Gene mining, characterization, and sequence alignment. Searching of gothic, Atg proteins in M. rosenbergii transcriptome dataset was carried out by using tBLASTn program in Essay on Child Disintegrative, CLC Main Workbench Version 6.0 (CLC Bio-Qaigen, AsiaPac, Taiwan) for which known Atg proteins (from mammals, yeast, and insects) were used as queries. The parameter was set as follows: matrix, BLOSUM62; e-value, 100. To search for gothic period art transcripts encoding Atg proteins in 21 century, other crustaceans, we used protein queries of known Atg proteins to search within publicly accessible databases [non-redundant (Nr), expressed sequence tags (ESTs), transcriptome shotgun assembly, and Daphnia genome [ 65 ] databases] by using NCBI tBLASTn program ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ), which was restricted to crustaceans (taxid: 6657) and the parameter was set as follows: expect threshold = 10; matrix, BLOSUM62; gap costs, existence = 11 and extension = 1. Nucleotide sequences obtained from BLAST searches were translated into amino acid sequences using the gothic period art CLC Main Workbench Version 6.0 and then primary sequence analyzed manually based on Essay Disorder, Asperger's Rett's Syndrome, homology to other known proteins. Protein alignment of crustacean Atg proteins with other previously known Atg proteins was performed using MEGA6 [ 66 ] and GeneDoc [ 67 ] softwares. Amino acid sequences used in the alignments, as well as their accession numbers, are provided in gothic art, Additional file 2 . All deduced M. rosenbergii Atg proteins were finally used for BLASTP search against the Nr protein database for further sequence confirmation. The BLAST hit with lowest e-value for each M. rosenbergii Atg protein is detailed in happened at three, Additional file 1 . Prediction of protein tertiary structure and critical sites. The tertiary structure of selected Atg proteins were predicted using the gothic art online tool Protein Homology/analogY Recognition Engine V 2.0 [ 68 ]. Essay And Citizenship. Visualization of predicted protein tertiary structure as a 3 dimensional model, illustration of hydrophobicity, superimposition of gothic period, proteins, and structure labeling were performed using the clothes UCSF Chimera version 1.8.1 [ 69 ]. The specific domains and critical sites were predicted by InterPro [ 70 ]. Primary antibodies used in western blotting and immunohistochemistry.
Rabbit polyclonal antibody against gothic BECN1 (Sigma-Aldrich, USA) was produced using synthetic peptide corresponding to amino acid positions 329–345 of spanish american war begins, human BECN1 as immunogen. Rabbit polyclonal antibody against LC3B (Sigma-Aldrich, USA) was raised against the human LC3B at amino acid positions 2–15. Rabbit polyclonal antibody against p62 (Santa Cruz, Biotechnology, USA) was produced against the human SQSTM1/p62 at amino acid positions 375–425. Art. Mouse monoclonal antibody against Lamp-1 (BD Science) was produced against human Lamp-1 at the amino acid positions 25–224. Western blot analysis of Atg8/LC3, Atg6/BECN1, and simon die in lord p62/SQSTM1. Fresh tissues were homogenized and sonicated in a lysis buffer (0.2 % NaDoc, 1 mM Na 3 VO 4 and 50 mM NaF) and then centrifuged at 10,000? g , 4 °C, for 1 h to obtain tissue extracts.
The supernatants were collected and gothic art protein concentrations measured with the clothes Bradford reagent (Sigma). For a positive control, human squamous carcinoma cells (A431 cell line) were sonicated in lysis buffer and protein concentrations measured. Subsequently, 30 ?g of gothic art, total proteins were separated on a 15 % SDS-PAGE gel and transferred onto a polyvinylidene fluoride membrane (GE Healthcare Life Sciences). Nonspecific binding sites were blocked with a blocking buffer [5 % non-fat dry milk in 0.2 % Tween 20 in 0.1 M phosphate-buffered saline (PBS), pH 7.4] for 2 h at 21 century clothes room temperature. Gothic Period. The membranes were incubated overnight at 4 °C with the 21 century following primary antibodies: either rabbit anti-LC3 (dilution 1:500), rabbit anti-p62/SQSTM1 (dilution 1:200), or rabbit anti-BECN1 (dilution 1:500). Period. The membranes were washed three times for 5 min each with washing buffer (0.01 % Tween 20 in life theories, 0.1 M PBS) and then incubated for period 2 h at room temperature in the peroxidase-conjugated secondary antibody [goat anti-rabbit IgG-HRP (SouthernBiotech) dilution 1:5000]. Antigen-antibody binding was detected using an enhanced chemiluminescent substrate (Thermo Scientific) and exposed to Amersham Hyperfilm ECL (GE Healthcare Life Sciences). Immunoperoxidase staining of Atg proteins in the ovary of M. rosenbergii. Immunoperoxidase staining was carried out following a previously described protocol [ 71 ] with some modifications.
Briefly, ovarian sections were sequentially deparaffinized and rehydrated in war begins, xylene and decreasing concentrations of ethanol. Endogenous peroxidase was blocked by immersing sections in gothic period art, 3 % H 2 O 2 in methanol for 30 min. Subsequently, the Essay sections were immersed in 1 % glycine in 0.1 M PBS to block free aldehyde groups and gothic period then incubated in a blocking serum (10 % bovine serum albumin in 0.1 M PBS, pH 7.4) for 2 h at 4 °C to block non-specific bindings. Sections were incubated overnight at 4 °C with either rabbit anti-LC3 (dilution 1:500), mouse monoclonal anti-Lamp-1 (dilution 1:500), rabbit polyclonal anti-p62/SQSTM1 (dilution 1:500), or rabbit polyclonal antibody anti-BECN1 (dilution 1:500), in blocking serum. After washing with PBS, sections were incubated in secondary antibody, either goat anti-rabbit IgG-HRP (Abcam) or goat anti-mouse IgG-HRP (SouthenBiotech) at course theories the dilution 1:500 in blocking serum, for 2 h at room temperature. Negative controls were performed by gothic period art, omitting the primary antibodies. The color was developed with an AEC substrate kit (Invitrogen). Finally, sections were observed and photographed under the Nikon E600 light microscope. BECN1, Beclin 1; Lamp-1, lysosomal-associated membrane protein 1Vps, vacuolar protein sorting; MAP1LC3, microtubule-associated proteins 1A/1B light chain 3; SQSTM1, sequestosome. This research was supported by a grant from the Office of 21 century, Higher Education Commission (OHEC) and Mahidol University (MU) under the National Research University Initiatives, an OHEC-MU and period art the Thailand Research Fund (TRF) Distinguished Research Professor Grant to Professor Prasert Sobhon, a TRF-RGJ Ph.D. Scholarships to Saowaros Suwansa-ard and Wilairat Kankuan; and an ARC grant to Disorder, Syndrome Dr.
Scott Cummins. We also thank the financial support from the art Consorzio InterUniversitario per le Biotecnologie (Trieste, Italy) and from the Associazione per la Ricerca Medica-Ippocrate-Rhazi (Novara, Italy) to Miss Wilairat Kankuan. This work was supported by a grant from the Essay on Equality and Citizenship Office of Higher Education Commission (OHEC) and Mahidol University (MU) under the gothic period National Research University Initiatives, an Essay and Citizenship OHEC-MU and gothic art the Thailand Research Fund (TRF) Distinguished Research Professor Grant to PS. SS was supported by a TRF-RGJ Ph.D. Scholarships. Spanish American War Begins. WK was supported by a TRF-RGJ Ph.D. Scholarship, the Consorzio InterUniversitario per le Biotecnologie (Trieste, Italy), and the Associazione per la Ricerca Medica-Ippocrate-Rhazi (Novara, Italy). SFC was supported by an ARC grant. Availability of data and materials. The datasets supporting the conclusions of this article are available in gothic period art, the NCBI Sequence Read Archive (SRA) depository (SRA Accession number: SRP049917; http://trace.ncbi.nlm.nih.gov/Traces/sra/?study=SRP049917 ). Course. Additional supporting data are included as additional files. SS performed the period art bioinformatics analyses and wrote the manuscript.
WK performed the Western blot analysis and how did simon of the immunohistochemistry, and wrote the manuscript. TT, JS, NK, TK and KK contributed the transcriptomic data and analysis. SFC, CI, and PS provided conception, laboratory support, critical revision of the manuscript, and gothic period final approval of the version to be published. The authors have declared that no competing interests exist. Ethics approval and Essay Disorder, Asperger's Disorder, consent to participate. Ethics is period, not required for the study. Open Access This article is distributed under the american war begins terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated. Saowaros Suwansa-ard 1 4 Wilairat Kankuan 1 5 Tipsuda Thongbuakaew 1 7 Jirawat Saetan 2 Napamanee Kornthong 3 Thanapong Kruangkum 1 Kanjana Khornchatri 1 Scott F. Cummins 4 Ciro Isidoro 5 Email author Prasert Sobhon 1 6 Email authorView author's OrcID profile 1. Department of Anatomy, Faculty of Science Mahidol University Bangkok Thailand 2. Department of Anatomy, Faculty of Science Prince of Songkla University Hatyai Thailand 3. Chulabhorn International College of Medicine Thammsat University Phathumthani Thailand 4. Period Art. Faculty of Science, Health, Education and Engineering University of the Sunshine Coast Maroochydore Australia 5. Laboratory of Molecular Pathology, Department of Health Sciences Amedeo Avogadro University Novara Italy 6. Essay On Child Disorder, Disorder, Rett's. Faculty of gothic art, Allied Health Sciences Burapha University Chonburi Thailand 7. School of Medicine Walailak University Nakhon Si Thammarat Thailand. .RIS Papers Reference Manager RefWorks Zotero. .BIB BibTeX JabRef Mendeley.
.RIS Papers Reference Manager RefWorks Zotero. .BIB BibTeX JabRef Mendeley. Over 10 million scientific documents at your fingertips. 2017 Springer International Publishing AG. Part of Springer Nature.
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8 Tips for Dazzling an Editor With Your Personal Essay. Ironically, as a reader, I never used to be a fan of period art anthologies or personal essay collections. As a teacher, I did love showing students how to write personal essays or short memoir pieces. As an English teacher and a writing instructor, it often felt miraculous to me how a mediocre piece could be transformed in just a few short weeks through revision, how a piece could evolve from bland and cliched to raw, powerful, and beautiful. But I never liked reading short pieces in my leisure time. It wasn't until I started writing as a blogger and spanish american freelance writer that I started to appreciate collections of personal essays as a genre.
I love seeing writers that I know online take different perspectives and approach topics with unique styles. As a parent, reading about other mothers' experiences from so many different angles has helped me gain insight into myself as a mother. Gothic! I've been thinking a lot about personal essays from three different perspectives: as a reader, as a writer, and now as an editor. I've been trying my hand at simon of the flies, publishing my own pieces, and I know that it's hard (really hard) to write a great personal essay. Gothic Period! After our call for submissions for My Other Ex: Women's True Stories of Leaving and Losing Friends , to be published in how did simon of the, September, I also spent months reading essays with an editor's eye, trying to decide which pieces to gothic period, accept and which to pass on. And that was just as hard. 21 Century Clothes! And it occurred to me as a beginning editor that we editors are not often transparent about what we are looking for. I'm lucky in the sense that I taught writing and developed writing curricula for well over a decade, and all of the best practices (and unwritten rules) of memoir and period essay writing are (somewhat) fresh in my mind. But most of us writers haven't taken an Essay Disorder, Rett's English class in quite a while. And we aren't recent MFA graduates either. So here's what I think -- as a teacher, writer, editor, and period reader -- about the mile ingredients of a great personal essay, one that is carefully crafted to draw in a reader, make her care about a topic, and keep reading.
1. Use what you know about good fiction and storytelling . You should develop characters, settings, and period art plot (a sequence of events) into a story. Use sensory details and vivid description to create separate, carefully chosen scenes. 2. Combine the personal and the universal . This is your story, your life, your emotions but your writing should also express and simon die in lord of the flies reveal a larger meaning, a theme, a deeper truth, beyond the surface details of plot and character. 3. Find your voice . More importantly, find your unique voice that is best for period art each piece, or different moments of the same piece. As Kate Hopper, in theories, the invaluable Use Your Words: A Writing Guide for Mothers, explains, voice is: the feel, language, tone, and syntax that makes a writer's writing unique. In nonfiction, voice is you, but not necessarily the you sitting in front of the gothic art computer typing away. Voice can be molded by a writer to serve the subject about which she is writing.
It might take a while to find the of the flies best voice for a piece. Is the right voice ironic, funny, anxious, playful, breathless, or solemn? We all have multiple identities and show different parts of ourselves at gothic, different times. Use that versatility in your writing. 4. Alternate focusing in and focusing out . Life! Choose specific and compelling moments, memories, and gothic feelings, and hone in on them, using those particular moments to help to convey theme and purpose. Pretend you are using a video camera to focus in and out, slowing down the on Child Disorder, Rett's action, like a cinematographer, very purposefully to guide the reader toward what's important in the piece. 5. Be specific, not general. Gothic! This is what I called The Rule of the Pebble to my students (thanks to Nancie Atwell, my writing teacher guru). Spanish War Begins! It basically means don't write about a general topic or idea; write about one particular person, place, time, object, or experience. Period Art! In other words, don't try to write about of the flies, all pebbles everywhere (or love or friendship or football or sunsets).
Write about this one particular pebble (or the friend that broke your heart freshman year, or the sunset that you saw last night, or memory, or place), its meaning to you, the concrete details that shape how you think about it. 6. E xperiment and play . Try out different literary devices and techniques, such as similes, personification, and metaphors. Or experiment with using different sentence lengths strategically. Use repetition, of words, of lines, of phrases. Play with imagery. Many of these devices should only be used sparingly, but, used effectively, they can add surprises and richness to your writing. 7. Gothic! Learn the difference between revision and clothes editing . You must do both. It's easy as a writer to focus on spelling errors and sentence structure, rather than making big (painful) changes to our writing. Gothic! Revision means to look again. You do things like: make sure that your theme and purpose for writing are clear; try out different leads (ways to spanish american war begins, begin the piece); rethink your conclusion; change the organization.
In editing, a separate stage, we do things like catch run-on sentences, fix errors in gothic period, punctuation or spelling, or replace overused words and american expressions. 8. Gothic! Read, read, read, and read some more . At Three Mile! What all writers have in common, as far as I know, is that they're constantly reading. They pay attention to gothic period, their favorite writer's craft and style and try them out in their own writing. They internalize the beauty and 21 century clothes the utility of the perfect word, the perfect sentence, and the perfect metaphor. What are your favorite personal essays?
Whose writing do you turn to as models for your own writing? To learn more about The HerStories Project and its next essay collection about friendship breakups, My Other Ex: Women's True Stories of Leaving and Losing Friends , visit the HerStories website.
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Example Of Narrative Essay Essays and Research Papers. boot-camp not as a military training program, but rather as a group of individuals who are driven by a common goal? If we can do this then we can begin . making comparisons with other groups of individuals and notice a great deal of similarities. For example , universities offer a wide variety of clubs such as the Philosophy Club or the Student Veterans Organization, and these clubs are formed because like-minded individuals driven by a common goal congregated and period, bonded to birth their club. Just like. Bond , Bonds, Lancashire , Marine 900 Words | 2 Pages. Pamela Essay 1 Sitting at my desk at home, a crushing feeling crept up on life theories me. I didn’t know what to think.
I’m not going to period art, make . it, am I. It was already midway through junior year of high school, and having just calculated my GPA requirements for many of the colleges I had wanted to go to, I realized that my grades were not even close to what I expected them to be. Of The Flies! I guess I had been in gothic period art, denial for so long that I let them slip this far. How could I have let this happen? Where was my. Debut albums , Disappointment , High school 1235 Words | 3 Pages. The Narrative Essay *What is how did, a Narrative Essay ? • Narrative writing tells a . story. Gothic Period Art! In essays , the narrative writing could also be considered reflection or an exploration of the life author's values told as a story. The author may remember his or her past, or a memorable person or event from period, that past, or even observe the present. • The author may write about: -An experience or event from his or her past. 21 Century Clothes! -A recent or ongoing experience or event. . Essay , Essay mill , Metaphor 1511 Words | 6 Pages. Narrative Essays : To Tell a Story There are four types of essays : Exposition - gives information about . various topics to the reader. Description - describes in detail characteristics and traits.
Argument - convinces the reader by gothic, demonstrating the truth or falsity of a topic. Narrative - tells a story, usually from one person’s viewpoint. A narrative essay uses all the story elements - a beginning and ending, plot, characters, setting and climax - all coming together to complete the. A Story , Essay , Essays 1444 Words | 5 Pages. How to life course, Write an Accomplishment Essay What are your three most substantial accomplishments, and why do you view them as such? — Harvard . Period Art! http://www.free- essay -writing-topics.com/index.php?page=mba-application-accomplishment-questions What is the most significant change or improvement you have made to Essay on Equality, an organization with which you have recently been affiliated?
Describe the process you went through to identify the gothic period art need for life course theories, change and manage the period process of implementing change. What were the results. Narrative , This Boy's Life , Writing 1811 Words | 4 Pages. Narrative Essay A Brief Guide to Writing Narrative Essays Narrative writing tells a . story. In essays the narrative writing could also be considered reflection or an exploration of the author's values told as a story. The author may remember his or her past, or a memorable person or event from that past, or even observe the present. When you're writing a narrative essay , loosen up.
After all, you're basically just telling a story to Essay, someone, something you probably do every day in casual conversation. Essay , Experience , Five paragraph essay 1314 Words | 5 Pages. As a mode of expository writing, the narrative approach, more than any other, offers writers a chance to think and write about themselves. We . all have experiences lodged in our memories, which are worthy of art, sharing with readers. Life Theories! Yet sometimes they are so fused with other memories that a lot of the time spent in writing narrative is in the prewriting stage. When you write a narrative essay , you are telling a story. Narrative essays are told from a defined point of view, often the author's, so there. Article , Essay , Essays 797 Words | 3 Pages. Sample SAT Essays —Up Close Below is our sample essay question, which is designed to be as close as possible to an . essay question that might appear on the SAT. You’ll recognize that it’s based on the great philosopher Moses Pelingus’s assertion, “There’s no success like failure,” which we have referred to throughout this chapter. Gothic Art! This particular essay topic presents you with a very broad idea and then asks you to explain your view and back it up with concrete examples . Not every SAT essay topic will.
Dot-com bubble , Essay , Essays 2405 Words | 7 Pages. Narrative Essay Vs. Descriptive Essay. ? Narrative Essays Are a Great Read Name ENG121 Professor June 16, 2014 Narrative . Essays Are a Great Read Narrative essays and Descriptive essays can be similar but they are different in war begins, nature. The narrative essay “I Want a Wife” is more compelling than the descriptive essay “Homeless” because the narrative essay has a point of art, view, uses humor and satire, and uses tone and language that can draw the reader in. “Narration is Essay on Child Disorder,, storytelling from the gothic perspective of. Essay , Essays , Homelessness 1604 Words | 7 Pages.
? Descriptive Essays vs. 21 Century! Narrative Essays Many people have different preferences on what type of writing style . they think is more superior to another, I believe descriptive writing to be more excellent writing style then narrative . I can tell you that there are a few similarities and a few differences between the two. I prefer Descriptive essays , rather than narrative essays . Gothic Art! In my belief, it's that the descriptive essays are more effective when an author is trying to convey a story or get a. Essay , Fiction , Narrative 1153 Words | 4 Pages. Structure of american, a Personal Narrative Essay. Writing Center Structure of a Personal Narrative Essay “ Narrative ” is a term more commonly known as “story.” . Narratives written for college or personal narratives , tell a story, usually to some point, to illustrate some truth or insight. Following are some tools to gothic period art, help you structure your personal narrative , breaking it down into parts.
The “Hook” Start your paper with a statement about 21 century, your story that catches the reader’s attention, for example : a relevant quotation, question, fact, or . First-person narrative , Grammatical person , Grammatical tense 789 Words | 5 Pages. About Narrative Essay Narrative essay is a popular topic on the Continuous Writing section and . students should take note that this topic has appeared in period, the SPM examination almost every year since the paper was introduced. Many people think that writing a story is a difficult task, but believe me, it is much easier than what you think because you are not confined to any particular point. Therefore, you are free to voice out everything that is in at three mile, your mind. A narrative essay is gothic period art, a piece of life, writing. A Story , Character , Essay 1134 Words | 3 Pages. joke about this accident. Things can seem horrible but really are insignificant moments in life. I can look back and laugh hysterically on a day I thought . was the period art end of the world. Narrative essay assessment rubric/score sheet Due date: __________________ Narrative essays must be typed, double spaced using 12 point font with a 1 inch margin. Headings must be left justified and include name, class period, title and section, assignment and how did simon die in flies, date, and be single spaced.
Titles. Middle school , Sensory 754 Words | 4 Pages. ?Composition II Eric Sack Fall 2014 Essay 1 - Narrative Argument Write an essay that implies a clear claim and uses . your own first-hand experience for support/evidence. The essay must use appeals involving logos, ethos and gothic, pathos, as well as connect with a general audience. For an 21 century clothes in-depth discussion of narrative arguments, see chapter 11 of Good Reasons. According to the book, narrative arguments rely on concrete individual stories rather than abstract statistics; they allow the gothic art readers to draw. Argument , Essay , Essays 723 Words | 2 Pages. Compare and Contrast Essay: Narrative and Descriptive Essays. Compare and Contrast Essay Name Institutional Affiliation Introduction Academicians argue that, a powerful reader paints a picture . on a reader’s mind.
Writing effective different types of essays is increasingly becoming a critical organ of academic success (Feng Checkett, 2014, p. 152). There are two major types of essays , narrative and descriptive. While the two might be appropriate in Essay, academic writing, one is art, arguably effective that the other. Narrative essays tells a story from Essay and Citizenship, personal. Essay , I Know Why the gothic period Caged Bird Sings , Maya Angelou 1036 Words | 6 Pages.
and situations.The very world itself would fall apart on the foundation that it was built on without literacy. What At Three Mile Island! The turning point in my literacy skills came . later on in my life through encouragment from my girlfriend and influence followed by the example set by my older brother Brian. During the gothic period summer program as a child, I practiced the alphabet and learn to read books and write at the first grade level. Countless hours doing repetitive excercises reading and how did simon die in, writing was not my cup of tea. Although. Essay , Full-time , High school 1159 Words | 3 Pages. Sample Essay Read this example sample essay . Then answer the gothic art questions below. Spanish American War Begins! The qualities of a hero . include selflessness, having the gothic period inspiration to spanish american, be a founder and being courageous.
With these qualities in mind, it is easy to see why many Emiratis see Sheikh Zayed as a hero. He embodied the three qualities mentioned above and these heroic qualities were seen in gothic period art, his life and work. He was born in Al Ain and had a simple education which included learning the Koran and mathematics. His. Abu Dhabi , Al Ain , Hero 1313 Words | 4 Pages. Romeo Teves EN101 Narrative Essay Professor Petro Fall 2012 Narrative . Essay : Early Morning Surfin’ ------------------------------------------------- Childhood is one of the most important things that make us who we are. These are the times when we learn certain things, do certain things and create certain things. Friends that we acquire while growing up seem to be the Essay Disintegrative Disorder, Disorder, longest lasting type of friends, versus friends that we make when we are in gothic, our late. Big wave surfing , Electromagnetic radiation , Surfing 1244 Words | 3 Pages. September 11th, 2012 Asmaa Salem Narrative Essay I am an average 16 year who just can’t wait to grow up and face the real . world and not have to worry about clothes, any rules or restrictions.
On January 12th, 1996, my parents named me Asmaa, so I have to stick with that name for the rest of my life. Personally, I like the name because it’s the name of a very great person; more of a hero in Islam. Names in my family go with a pattern; but this only goes for girls. Gothic Art! The pattern is the double ‘A’ at the. 2008 albums , Debut albums , Frustration 1565 Words | 4 Pages. NARRATIVE ESSAY PROMPTS [Being Unprepared] Because you have been sick, out of town, busy at work, or working on other . homework, you didn't have as much time to study for an important test as you needed. Spanish American War Begins! Everyone going to school has been in this situation. Think of art, a specific test that you took that you felt unprepared for and narrate the course events.
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Since we were seniors, we used to participate in the morning act on fridays, as usual for. Blushing , Embarrassment , Humiliation 778 Words | 3 Pages. ?Anthony Santos September 18, 2013 Narrative Essay Professor Wang Unfamiliar Territory I recall the nerves, . sweat, and some tears of my first day of public school in happened island, an unfamiliar environment. I was vulnerable and invisible to all the chaos occurring in gothic art, my peripheral vision. All I remember is seeing an endless sea of faces from all different colors, backgrounds, and Essay on Child Disintegrative Disorder, Asperger's Disorder,, ages. In the moment, I perceived that anyone who survived this madness would be considered invincible. This experience. Cafeteria , High school , Private school 825 Words | 3 Pages.
? NARRATIVE ESSAY NORMAL AGING OR ALZHEIMER’S? ENGLISH 101 DUE: 11-03-13 BY MELVA NORZAGARAY Normal aging or Alzheimer’s ? . I am never going to forget the beginning of the most important lesson life has taught me up to this date. Gothic Art! It was a rainy day. The sky was gloomy and the air was blowing cold. That day was very important to me, because my mother had an appointment with the Neurologist. It was the Essay on Equality first time she would visit him. She did not know why we were going to see him. Alzheimer's disease , Caregiving and dementia , Dementia 896 Words | 5 Pages. Achieving the American Dream Gentle, Revised Narrative Essay One would think that purchasing your first home would be a very . fascinating experience; after all it is one of the most major purchases of your entire life. In America, home ownership ties in with the American Dream and the spirit of working hard to one day earn through hard work a home with a white picket fence. Gothic Period Art! My husband and I felt that it was time for us to 21 century clothes, reward ourselves for all the hard work we have been putting in over the.
American Dream , Emily the Strange , Fence 1287 Words | 4 Pages. Donna Franklin Composition I 3 February 2013 Narrative Essay Significant components of art, life can happen at any time, . anywhere. Most times people don’t know when they happen until years later when those tiny little things lead into spanish american, something much bigger. Things can be as simple as meeting someone in school one day to getting a random text at your grandmother’s funeral viewing. Though they can be odd and something one would never expect in art, a million years, they can also be subtle enough to lead. Family , Friendship , Grandparent 1224 Words | 3 Pages. [pic] Faculty Of Entrepreneurship and on Equality, Business Group : SAK 1_1 UBI 1022 – English Language Mdm.
Lena Ramamurthy Narrative . Essay – My First Day In University Of Kelantan Name : Lim Wee Kiat Matric number : A10A249 Date of submission : 09/01/2011 Narrative Essay – My First Day In University Of Kelantan Perplex, that is the feeling that I have when I first came to University Malaysia of Kelantan (UMK). Gothic Art! Actually I never thought that I will be coming here to further my higher. Bus stop , Kelantan , Kota Bharu 1560 Words | 4 Pages. ?Wilson Guaman English 101-P Narrative Essay Life is full of course, surprises and difficulties. A twist of fate can make your . life miserable or blooming as the spring flower. When my brother and art, I used to live in Ecuador, everything was so amazing. We always used to be so close to each other. Our relationship as brothers was unique and 21 century, wonderful. I remember we used to go play soccer together every Friday, Saturday, and art, Sunday. We were actually the best soccer players that you can find. But my.
American films , English-language films , Family 1102 Words | 3 Pages. ?Sarah Bublitz 03/26/2014 Narrative Essay Have you ever had someone you had a rocky relationship with that doesn’t leave . you alone? Well, I have. I’m divorced from this man and he still continues to bother me. He is always around and I can’t get rid of him. You might ask me why and course, I’ll tell you. Period Art! It’s because we have two beautiful daughters together and I have to be an adult and still communicate with him about our children. Island! Why can’t he be an adult, I have no idea. I’m still trying. Marriage 1062 Words | 2 Pages.
Narrative Essay In a persons life friendship is gothic, a bond of lord flies, truth and faith. Friendship is gothic, a feeling of how did simon of the, love and gothic art, affection of . one person for another. Friends are very important part of life. On Child Disorder, Asperger's Rett's Syndrome! They are there to comfort, to laugh and to make memories with. My friend is very dear to me. We used to see each other early morning before our class starts.
The rest of the period art day passed awesome after meeting her. Life Course! After three years when my family and gothic period art, I came back to New York, I saw my friend one day on Main street. College , Debut albums , English-language films 1007 Words | 3 Pages. Narrative Essay First Grade I didn’t realize my specialness at the time, even though I didn’t know much English the first day . of school there. All the happened kids and period art, teachers were nice to me and well-mannered with good intentions, but they could clearly see how I was an outsider, and how I was different from Essay on Equality and Citizenship, them. I remember that I went to gothic period, the pool every weekend with my grandpa, and my parent’s started making me take piano lessons. Essay On Equality And Citizenship! Second Grade I was thrown into a completely new environment with. Education , Educational years , Elementary school 974 Words | 3 Pages. ?My Narrative Essay I believe that “Love at first sight” is period, real. What At Three Mile Island! An actual feeling you get when you first lay eyes on that one . special person. More special than anybody you’ve ever met.
The person that your heart just immediately falls in period art, love with and decides that it wants to life course theories, spend eternity with that person. Art! I believe in “Love at clothes first sight” because I’ve experienced in before. The feeling it gives you is period, like no other feeling you’ve ever felt before or ever experienced before in your life. Clothes! You. A Little Bit , At First Sight , Blond 1019 Words | 3 Pages. me because of these experiences. I will remember these experiences for gothic period, the rest of theories, my life because they each taught me so much. Dear Austin . Gothic Art! Baack, You have successfully submitted the file Triple Crown Award to the assignment Narrative essay in the class 2nd Hour Ginder World Lit. on at three mile island 06-Nov-2014 12:35AM.
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Still on theories the stage from giving a speech and thanking her parents for “helping her through the years”, Julia rushed to go hug them as they congratulated her on gothic art her success. Anti-obesity medication , Fenfluramine , Girl 1229 Words | 7 Pages. The Butterfly Effect Narrative Essay. Alyssa Iannotti Art of The Moving Image Narrative Essay November 28, 2007 The Butterfly Effect . Narrative form is simply “a type of filmic organization in Disintegrative Asperger's, which the parts relate to one another through a series of casually related events taking place in time and space.” Narration usually occurs in gothic period, a cause-effect relationship. There are many factors of spanish american war begins, narration but range and depth are the gothic period art most important. The range refers to how much we know in the plot. Range connects characters and. Ashton Kutcher , Eric Bress , Film 1208 Words | 3 Pages. ? Narrative Essay Wow, senior year of High School could not have gone faster.
It felt as if I blinked and three years of high . On Equality And Citizenship! school passed by. Everyone was worried about college acceptances and I was just worried about art, enjoying the last year of 21 century clothes, high school with my friends as it came to a close. Football season was the best time of the gothic art year as we practiced every morning at six. Our High School had won twelve state championships in the last thirteen years and Essay on Child Disintegrative Asperger's Disorder,, we were prepared to win another one that. American football , Anterior cruciate ligament , College 1071 Words | 3 Pages. August 28, 2008 English Narrative Essay My Unplanned Weekend It was a Thursday afternoon and I was patiently . sitting in my chair at school talking to my friend Tanya about what we were going to do that weekend. Gothic Period Art! I hear a voice say “Mr. Johnson can you send Lynn to the office for check out.” I was excited because this meant I did not have to go to math class. Course Theories! As I arrived at period the office, I saw my sister she looked as if something was wrong. “Kimberly, what is what mile, wrong?” “I will tell.
English-language films , Family , Grandparent 989 Words | 3 Pages. age of expansion following on an age of discovery, its expansion led to still further discovery about architectural design and decoration. Section A: . Interiors S.Maria Della Salute (1631-1682) The interior of S.Maria Della Salute is period art, a very good example of Baroque Architecture and Essay on Child Disorder, Disorder, Syndrome, design. It displays the Baroque essence in a way but is not completely over ornamented nor does it contain any unsuitable details. Marble is mainly used in gothic period art, the columns and the base appears to be gilded in bronze. Sculptures.
Baroque , Baroque music , Dome 1264 Words | 4 Pages. Kevin Heart . 4-3-12 English-110 Narrative essay Murder is the unlawful killing of another human being with malice afterthought, and generally this state of mind distinguishes murder from other forms of unlawful homicide (such as manslaughter). As the mile loss of a human being inflicts enormous grief upon gothic, the individuals close to the victim, as well as. Capital punishment , Capital punishment in life course theories, the United States , Death 1056 Words | 3 Pages. within a narrative are very important.
They help set the gothic art stage so the reader can understand the story from the author’s perspective. Essay On Child Disintegrative Disorder, Syndrome! A . narrative is structured so the reader can see things clearly. The structure contains the gothic art introduction, the body, and the conclusion. 21 Century! The introduction has a thesis statement. A thesis statement is a sentence that states the main point of the narrative . The introduction will clarify the main points the period writer will make throughout the body of the narrative . Simon Lord Of The Flies! The body. Automobile , Bicycle , Causality 1419 Words | 4 Pages. Sarah Parker Eng 102 Narrative Essay Degree Choice Everyone in the world has a journey to find their passion. Whether it . Gothic Period! be short, long, thought-out or impulsive, people may find out what career field they'd like to join.
Once one discovers their passion and what career path to follow, they are then made to spanish american, pursue a degree that will help them excel. For some it took only art moments to decide their fate; it takes some only a simple decision towards what path they want to take. It took me years. Academic degree , Associate's degree , Bachelor's degree 1423 Words | 4 Pages. K. Rogers 2-26-13 Crazy Canine Catastrophe narrative essay There was a calming familiarity of the obnoxious clanging . of pots and spanish war begins, pans in the kitchen that morning, as I began to wake up to gothic period, the sight of another seemingly unrelenting snow storm.
I wiped off the greenish, yellow crust that had built up around my eyes from the on Child Disintegrative Asperger's Disorder, night before and glanced out art through the Essay Disintegrative Asperger's Disorder, iced-over window pane in my room. I couldn't see further than fifteen yards in period, any direction, barely being able to make out. Mother 1761 Words | 4 Pages. Name: Jamin Clubb English 1101 Class 425 9/20/11 Narrative essay I remember Valentine’s Day this past year like no other; . Essay! it was on a Monday this year. Mondays for my wife and me were like our Fridays, we both worked weekends, and for years never got to go out gothic art much; that was until we discovered half priced beers at what at three island a place called World of Beer. That became our normal spot; we even got a large group of friends to come out with us every Monday night for drinks and gothic period art, games at the bar. About a.
Debut albums , Doctor , Human chorionic gonadotropin 1124 Words | 3 Pages. English 101 Narrative Essay In Death, You Live Forever “Can you get me a glass of water?” my mother whispered in theories, a hoarse . Gothic Period Art! voice. How Did Simon Lord Of The Flies! I nodded and quickly escaped the dimly lit bedroom to gothic, fetch my mother a glass of water from the 21 century clothes kitchen. She said she wanted water, and I believed her one hundred percent, but I knew that she had another motive for gothic period art, sending me out what mile of the gothic art room. 21 Century Clothes! She wanted to speak to her friend, Angelo, in private. I knew she would be talking to him about her condition, but that’s.
Barbie , Bild Lilli doll , Book 1519 Words | 4 Pages. Camacho Narrative Essay Week 5 Revision There can be major physical changes and effects to art, a female’s body after having a . child. In 2011 when I had my son the hospital gave me an epidural for the pain; they did the Essay on Equality shot too high up on my back. As far as anyone knew I was fine. I showed no signs of problems until after I got out of the gothic period art hospital, when I started having back spasms due to the fact that the epidural was done incorrectly. The female body goes through many natural changes; it prepares. AIDS , Bile , Bile duct 1154 Words | 3 Pages.
Compare-Contrast Essay Eng121: English Composition I (AXC13480) Regina McKinney Professor: Nancy Segovia January 1, 2014 A . narrative essay is simon die in of the, about storytelling for a narrative story to work it must capture and hold the audience attention you must give a clear understanding of your story. A descriptive essay lets you describe in detail what the essay is all about using words that appeal to gothic period art, your sense of smell, hearing, see, touch, and taste. A descriptive essay lets you use words that. Essay , Maya Angelou , Narrative 1226 Words | 4 Pages. SECRET TALENT When we talk about talent then first question that comes in our mind is “what is talent?” .Talent is any natural ability or power of a . 21 Century Clothes! person. Talent actually means that someone has special ability or power in some specific field. For example someone has power to change the gothic period peoples mind through his/her speaking power it means that that person has speaking ability and power in his/her voice that can affect the on Child Disorder, Disorder, Rett's Syndrome peoples mind. It’s called talent.
Talent is natural ability it builds self-confidence. Billboard Hot Dance Club Songs number-one singles , Cognition , English-language films 760 Words | 3 Pages. old skills. Been a volunteer can help many people in period, the world. Spanish American War Begins! Such as people who are in the hospitals suffering from gothic, sicknesses that they no cure also . people who are without family and Essay on Equality, many kids that are suffering from very bad sicknesses for example , cancer IDS. Gothic Period! Many people who have cancer and IDS are the most needed suffer people in the hospital according to the National Cancer Institute cancer and happened at three mile, IDS affect the whole family not just the person with the disease.
These means that people who. Hospital , National Cancer Institute , Volunteer 1429 Words | 4 Pages. An Example Of Narrative Animation. ?An Example of art, Narrative Animation: Duck Amuck During the golden age of Hollywood short cartoons, from the 1930s to the 1950s, . Clothes! Disney and Warner Bros. were rivals. Disney animators had far greater resources at their disposal, and gothic period art, their animation was more elaborate and detailed than the Essay on Child Disintegrative Disorder, Asperger's Disorder, Rett's simpler style of the Warner product.
Warner cartoonists, despite their limited budgets, fought back by exploiting the comic fantasy possible in art, animated films and playing with the medium in Essay Disintegrative Disorder, Rett's Syndrome, imaginative ways. Gothic Period! In Warner. Animation , Bugs Bunny , Chuck Jones 1216 Words | 3 Pages. Examples from spanish american, Reflection Essays Disciplinary Awareness “The research I did this summer focused on sorption of gothic art, cesium and . strontium by soils. Asperger's Disorder, Syndrome! These two chemicals are commonly found in chemical contaminants… My research also focused on the distribution coefficient which is a measurement of how much of a solvent is [absorbed] by a geologic medium.” “I found that many of the readings I came across regarding abortion seemed to convey a strong belief in human rights, which was demonstrated by the dichotomy.
Abortion , Clean Water Act , Human rights 1543 Words | 5 Pages. Example Essay “He that is gothic period art, good for 21 century clothes, making excuses is gothic period art, seldom good for anything else.” -- Ben Franklin. As early as the . founding of the United States of America, Mr. Franklin observed society using the excuse, I don't have enough time… and it negative effects on their lives. Happened At Three Mile Island! Today, it is gothic art, frequently used as an excuse to justify the theories lack of gothic, time management skills. The effects on kids, work, or even in family life are sometimes devastating. Essay And Citizenship! In a day there are 24 hours, and time is gothic art, available. Benjamin Franklin , Family , Franklin Planner 950 Words | 3 Pages. surprised to find three judges, different from the previous year. What Happened At Three Mile Island! My face literally turned as bright as the sun when I did not see the judge that absolutely . detests my teacher, *a*c* *e*s*n (for privacy reasons, I will exclude her name from this essay ). I turned as hyper as a kid could become, and I was about to scream from gothic art, happiness right then.
Every single time I am in a competition, and she’s a judge, she always marks me down. Luckily, she was not here this time, and instead, sitting in life, her. Competition , Debut albums , Mess 1337 Words | 4 Pages. idea of ghosts is far too exaggerated to gothic art, be real. According to psychologydictionary.org the paranormal is “designating any phenomenon comprising the . transfer of data or energy which can't be described by present scientific insights” (“What”). For example , a couple of years ago I was in die in lord of the, my house alone. Period! I was walking towards the front door and a pair of scissors fell from the countertop and on on Equality to the art floor. 21 Century Clothes! It scared me to death and it was like the scissors had been thrown. Period! I do not know if it was.
Cryptid , Ghost , Loch Ness Monster 1320 Words | 5 Pages. mess you up for life. Therefore these experiences will become a necessary example to teach you how to deal with the unfairness . in how did simon lord of the flies, life. When I first started middle school I felt so out of place, I mean I had to deal some very flaky people and I was very stressed out a lot. Gothic Period Art! The reason why I was so stressed out was because of the changes that I had to endure around and to Essay, me. Gothic Period! For example I had to deal with the disturbing fact that I was growing hair in various places on my. College , English-language films , High school 1128 Words | 3 Pages.
? Eng 121 Narrative Essay 02/13/2014 Education is the key to success and as such the only way to get to the top. Lord Of The Flies! As the art . Essay Disintegrative Disorder, Asperger's Disorder, Syndrome! late president Nelson Mandela said, “It always seems impossible unless it’s done. I was fourteen years of age when this incredible opportunity came for me to join the Bournvita brain match quiz club sponsored by Cadbury as a representative for my junior high school. Period! On my journey to how did simon flies, the quiz club, I learnt how to associate with people, listen and gothic, not. College , Grammar school , High school 1573 Words | 4 Pages. Keba Raye Eng 101 (online) Narrative Paper / Final Draft A DANCER WAS BORN I had only hours until those big red curtains slowly dragged . apart. Essay Asperger's Disorder, Syndrome! I had been practicing for gothic art, the most important showcase of the life theories year for over a month now. I t was my first solo performance and my nerves were rattling. I was only nine! How was I supposed to handle the most important part of the gothic period art entire routine all on my shoulders? I stared around at the gym.
Today it seemed extremely larger than any other day. All. Dance , Lunch , The Showcase 897 Words | 3 Pages. ?John Doe Professor Mansito ENC 1101 12:00-1:50 21 October 2013 Essay 1 Sexuality: The 1950’s to spanish war begins, Today Sexuality, like many other . Period Art! things in our world, is an ever changing thing. The ideas and connotations surrounding it change from spanish american war begins, generation to generation. Because of this, the period idea of sex in life course theories, the 1950’s is gothic, completely different from the spanish american war begins idea of sex today. Gothic Period! Today, sexuality can be expressed in almost anything we do.
Commercials, billboards, TV shows, movies, magazine articles, and many other. 20th century , 21st century , Global warming 1105 Words | 4 Pages. ?Alena Professor Okun ENC 1101 3 February 2014 Narration Essay Number One Growing up nothing ever affected me as much as the time when I . saw my baby sister in spanish, agony. Cecelia was only gothic art two years old at the time and spanish, yet she had already gone through something so horrific and frightening for a toddler to period art, experience. I could not understand what was happening as I was only a little girl. The feeling that I had in the pit of my stomach left me feeling petrified because it was truly one of my worst. Advanced Trauma Life Support , Burn , Doctor 1667 Words | 4 Pages. other areas) show specific anatomical and spanish war begins, functional features in professional and semi-professional musicians.” One of the most visible . benefits of playing a musical instrument is the gothic improvement of memory capacity and ability. What At Three Mile Island! For example , the constant use of both the left and right hemispheres of the brain simultaneously in someone who plays the piano exercises memory.
Thanks to this, it is also believed that people who play musical instruments are less prone to have neurodegenerative. Brain , Intelligence , Intelligence quotient 946 Words | 3 Pages.